Evaluation of a model for studies on sequelae after acute otitis media in the Mongolian gerbil.

Abstract

CONCLUSIONS: The model appears relevant for studies on sequelae after acute otitis media (AOM), and may be the seed of a new, chronic tympanic membrane perforation model in the gerbil. OBJECTIVES: To evaluate an experimental model for abortive otitis media and to assess the structural and functional changes of the tympanic membrane in the resolving phase. MATERIALS AND METHODS: The middle ears of 16 Mongolian gerbils were inoculated with type 6a Streptococcus pneumoniae. Half of the animals were treated with antibiotics on days 4-6, when otoscopy was performed as well. After 1, 2, 3 or 4 weeks the animals were sacrificed and their tympanic membranes were examined by otoscopy, dissection microscopy, light microscopy and moire interferometry. RESULTS: On days 4 and 6 AOM was produced in approximately 80% of the animals and perforations prevailed in approximately 30% at the study end points. Clinical signs of AOM and oedema of the tympanic membrane had already started to reduce after 1 week, and often resolved within 2 weeks. The mechanical stiffness of the tympanic membrane remained relatively unharmed in the non-perforated ears. The antibiotic treatment seemed to reduce the duration of oedema but not the perforation rate.