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Peer-reviewed veterinary case report

Testing clopidogrel response in dogs with a platelet function analyzer

By Shin, Joon-Ho & Han, Hyun-Jung·Published in Frontiers in veterinary science·2025·Department of Veterinary Emergency and Critical Care Medicine, South Korea·View original on PubMed

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Original publication title: Evaluation of clopidogrel responsiveness using the Platelet Function Analyzer-200 (PFA-200) in dogs.

Species:
dog

Plain-English summary

A group of hypercoagulable dogs (dogs with increased blood clotting) was tested for how well they responded to a medication called clopidogrel, which is used to prevent blood clots. Out of 30 dogs, 23 responded well to the standard dose, while 7 did not. For those 7 dogs that showed resistance, increasing the dose of clopidogrel helped them respond better. The study also found that measuring D-dimer levels could help predict which dogs might not respond to the initial treatment.

People also search for: dog blood clot treatment · clopidogrel resistance in dogs · dog D-dimer levels test

Abstract

We aimed to evaluate the prevalence of clopidogrel resistance in hypercoagulable dogs using the Platelet Function Analyzer-200 (PFA-200) P2Y cartridge; further, we aimed to assess the utility of hematocrit (HCT), platelet count (PLT), prothrombin time (PT), activated partial thromboplastin time (aPTT), thromboelastography (TEG) parameters, and D-dimer level as indicators of clopidogrel efficacy. Forty healthy dogs underwent single measurements of P2Y closure time (CT), HCT, PLT, PT, aPTT, TEG parameters, and D-dimer levels, while thirty hypercoagulable dogs underwent two measurements of these parameters before and after clopidogrel treatment. The reference interval for P2Y CT in healthy dogs was 40.0-141.5&#x202f;s, with a mean of 63.9&#x202f;&#xb1;&#x202f;26.82&#x202f;s. Hypercoagulable dogs showed a mean baseline P2Y CT of 77.4&#x202f;&#xb1;&#x202f;37.6&#x202f;s. Moreover, 23 (76.67%) and 7 (23.33%) showed responsiveness and resistance to the initial clopidogrel dose, respectively. The mean P2Y CT of the clopidogrel-resistant group after clopidogrel administration was 182.71&#x202f;&#xb1;&#x202f;78.43&#x202f;s. Increasing the maintenance dose successfully overcame clopidogrel resistance in these seven dogs. Among the assessed parameters, only D-dimer levels showed a significant decrease in the clopidogrel-responder group (&#x202f;<&#x202f;0.05), suggesting its potential utility in evaluating responsiveness. In conclusion, the PFA-200 P2Y cartridge effectively detects clopidogrel resistance in dogs and can guide therapeutic adjustments such as dose escalation.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/40717918/