Peer-reviewed veterinary case report
DNA methylation of ABCB1 gene studied in dogs with lymphoma
By Tomiyasu, Hirotaka et al.·Published in American journal of veterinary research·2014·Department of Veterinary Internal Medicine, Japan·View original on PubMed →
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Original publication title: Evaluation of DNA methylation profiles of the CpG island of the ABCB1 gene in dogs with lymphoma.
- Species:
- dog
Plain-English summary
A group of 27 dogs with high-grade lymphoma, a type of cancer affecting the lymphatic system, were studied to understand the role of a specific gene (ABCB1) in their tumors. Researchers found that the gene's activity was not significantly affected by changes in DNA methylation, which is a process that can regulate gene expression. This means that the ABCB1 gene was still active in most dogs, regardless of whether they responded well to chemotherapy or not. The findings suggest that other factors may influence how these dogs respond to treatment.
People also search for: dog lymphoma treatment · chemotherapy for dogs with cancer · ABCB1 gene in dogs
Abstract
OBJECTIVE: To examine the DNA methylation status of the ABCB1 gene in tumor cells of dogs with lymphoma. ANIMALS: 27 dogs with multicentric B-cell high-grade lymphoma (19 chemotherapy-sensitive dogs and 8 chemotherapy-resistant dogs). PROCEDURES: The DNA methylation profile of the CpG island of the ABCB1 gene was analyzed by use of bisulphite sequencing and real-time methylation-specific PCR assay in lymphoma cells. Quantitative reverse transcriptase PCR assay of the ABCB1 gene was conducted to measure the amount of mRNA. Correlation between the amount of ABCB1 mRNA and the methylation rate was examined. RESULTS: The CpG island of the ABCB1 gene was hypomethylated in most dogs in both the chemotherapy-sensitive and -resistant groups. No significant difference was detected in the methylation rate between the 2 groups, and no significant correlation was detected between the methylation rate and the mRNA expression level. CONCLUSIONS AND CLINICAL RELEVANCE: Expression of the ABCB1 gene was not suppressed by hypermethylation of its CpG island in most dogs with lymphoma regardless of their chemotherapy sensitivity status.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25157887/