Peer-reviewed veterinary case report
Immunohistochemistry improves diagnosis of sporotrichosis in dogs
By Miranda, Luisa H M et al.·Published in Veterinary journal (London, England : 1997)·2011·Laborató, Brazil·View original on PubMed →
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Original publication title: Evaluation of immunohistochemistry for the diagnosis of sporotrichosis in dogs.
- Species:
- dog
Plain-English summary
A group of dogs with sporotrichosis, a fungal infection, was tested using a special staining method called immunohistochemistry (IHC) to see how well it diagnosed the condition compared to other techniques. Out of 87 dogs with sporotrichosis, IHC was able to detect the fungus in 65.5% of cases, which was more effective than the other methods used. This means IHC could help vets diagnose sporotrichosis more accurately. The study suggests that using IHC could improve diagnosis and treatment for affected dogs.
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Abstract
The aim of this study was to apply immunohistochemistry (IHC) for the diagnosis of canine sporotrichosis and to compare this method with the Grocott's silver stain (GSS) and periodic acid Schiff (PAS) techniques. Eighty-seven dogs with sporotrichosis (group 1) and 35 with American tegumentary leishmaniosis (ATL) (group 2) were studied. The fungus was detected in group 1 by GSS, PAS and IHC. IHC was also applied to group 2 to evaluate the occurrence of cross-reactions. PAS, GSS and IHC detected yeast cells in 19.5%, 43.7% and 65.5% of the group 1 cases, respectively. The detection of intracellular antigens of Sporothrix schenckii by IHC increased the sensitivity of the histological diagnosis to 80.5%. No positive reaction was observed in ATL lesions. The results suggest that IHC may be indicated for the diagnosis of sporotrichosis because of its higher diagnostic sensitivity.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21216636/