Peer-reviewed veterinary case report
Tests using Leishmania infantum antigens to detect leishmaniasis
By Pinedo-Cancino, Viviana et al.·Published in Acta tropica·2016·Instituto de Medicina Tropical de Sã, Brazil·View original on PubMed →
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Original publication title: Evaluation of Leishmania (Leishmania) infantum excreted-secreted antigens for detection of canine leishmaniasis.
- Species:
- dog
Plain-English summary
A group of 155 dogs was tested for canine leishmaniasis, a disease caused by a parasite. Out of those, 100 dogs were suspected to have the disease, and blood tests confirmed that 54% were indeed infected. The tests used, including ESA-blot and ELISA, were effective in detecting the infection, even in dogs that showed no symptoms. The results showed that these tests could accurately identify infected dogs, helping veterinarians diagnose and treat the disease more effectively.
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Abstract
The efficacy of tests with L. (L.) infantum excreted-secreted antigens (ESA) to detect canine leishmaniasis (CanL) was evaluated using immunoblotting (ESA-blot), ELISA (ESA-ELISA) and ELISA with alkaline extract from promastigotes (PAE). Of one hundred fifty-five domestic dogs tested, 100 were suspected of CanL, 23 had other diseases and 32 were healthy. Sera from the dogs suspected of CanL were tested by immunohistochemistry (IHC), and 54% were confirmed to be infected by L. (L.) infantum (38 symptomatic and 16 asymptomatic). Of these, 100% were positive by ESA-blot, ESA-ELISA and PAE-ELISA. In the ESA-blot their sera recognized polypeptides in the 26.5-31.5kDa region. Of the 46% of dogs with negative IHC, 44-53% tested positive in all three tests irrespective of clinical status. The twenty-three dogs with other diseases were negative by ESA-blot, but sera from 9% and 26% of them reacted with ESA-ELISA and PAE-ELISA, respectively. The 32 healthy dogs were negative in all the tests. ESA-blot showed good correlation with IHC in the detection of CanL and a high specificity index.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/27212707/