Peer-reviewed veterinary case report
Evaluation of the combined use of the recombinant Brucella abortus Omp10, Omp19 and Omp28 proteins for the clinical diagnosis of bovine brucellosis.
- Journal:
- Microbial pathogenesis
- Year:
- 2015
- Authors:
- Simborio, Hannah Leah Tadeja et al.
- Affiliation:
- Institute of Animal Medicine · South Korea
Plain-English summary
This study looked at a new way to diagnose brucellosis, a bacterial infection in cattle, by using specific proteins from the bacteria instead of the usual methods that can confuse vaccination with active infection. Researchers tested these proteins on blood samples from Korean native cattle and found that they worked really well, correctly identifying most of the infected animals. The combined proteins showed a high level of accuracy, sensitivity, and specificity, meaning they were very effective at distinguishing between infected and non-infected cattle. While these findings are still early, they suggest that this method could be a promising tool for diagnosing brucellosis and might even help in creating better vaccines in the future.
Abstract
Currently, there are several serodiagnostic tools available for brucellosis, however, it is difficult to differentiate an active infection from vaccination. Hence, there is a great need to develop alternative means that can distinguish between these two conditions without utilizing lipopolysaccharide (LPS). This study was an attempt to determine the efficacy of combined recombinant Brucella (B.) abortus outer membrane proteins (rOmps) and individual rOmps in the serodiagnosis of brucellosis by enzyme linked immunosorbent assay (ELISA), utilizing both that standard tube agglutination test (TAT)-positive and -negative serum samples from Korean native cattle. The results are very interesting and promising because the combined rOmp antigens used in the study were highly reactive with the TAT-positive serum samples. The combined rOmps sensitivity, specificity and accuracy were 215/232 (92.67%), 294/298 (98.66%) and 509/530 (96.04%), respectively. While these results are preliminary, the tests performed have very high potential in the serodiagnosis of brucellosis and likewise, the combined rOmps can be used for future vaccine production.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25988974/