Peer-reviewed veterinary case report
MYC_CANFA gene study in Shar Peis with low vitamin B12
By Grützner, Niels et al.·Published in Veterinary clinical pathology·2013·Department of Small Animal Clinical Sciences, United States·View original on PubMed →
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Original publication title: Evaluation of the MYC_CANFA gene in Chinese Shar Peis with cobalamin deficiency.
- Species:
- dog
Plain-English summary
A group of Chinese Shar Peis with cobalamin (vitamin B12) deficiency was studied to see if there were any mutations in a specific gene that could explain their condition. Researchers looked at the MYC_CANFA gene and found no significant differences between the affected dogs and those with normal cobalamin levels. This suggests that the deficiency is not caused by mutations in this gene. More research is needed to identify the exact cause of cobalamin deficiency in this breed.
People also search for: Chinese Shar Pei cobalamin deficiency · dog vitamin B12 deficiency symptoms · Shar Pei genetic health issues
Abstract
BACKGROUND: A recent genome-wide scan using the canine minimal screening set 2 (MSS-2) showed that cobalamin deficiency appears to be hereditary in Chinese Shar Peis and is linked to the microsatellite markers DTR13.6 and REN13N11 on canine chromosome 13. OBJECTIVE: The goal of this study was to evaluate the MYC_CANFA gene, which is the closest known gene with a distance of approximately 0.06 megabases (Mb) to the microsatellite marker DTR13.6, for any mutations in this breed. METHODS: Microsatellite markers (Myc and G15987) for genotyping and primers for sequencing were used to evaluate the MYC_CANFA gene. The genotype and gene sequence were compared between cobalamin-deficient Shar Peis, Shar Peis with normal serum cobalamin concentrations, and the DNA sequences published as part of the Ensemble Genomic map. RESULTS: Neither the microsatellite markers (Myc and G15987) nor the sequences of the MYC_CANFA gene showed a significant difference among both groups of Shar Peis and the published canine DNA sequence. CONCLUSIONS: The data presented here suggest that cobalamin deficiency in Shar Peis is not related to any mutations of the MYC_CANFA gene according to the genotyping and sequencing results in this study. Further investigations are warranted to find a potential genomic locus in proximity to DTR13.6 and REN13N11 that shows mutations in cobalamin-deficient Shar Peis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23278253/