Peer-reviewed veterinary case report
First molecular detection of Trypanosoma evansi in dogs from Paraguay
By Oscar Daniel Salvioni Recalde et al.·Published in Brazilian Journal of Veterinary Medicine·2021·Biologist, Molecular Biology Department, Centro para el Desarrollo de la Investigación Científica (CEDIC), Asunción, Paraguay, BR·View original on DOAJ →
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Original publication title: First report of the presence of Trypanosoma evansi in dogs from Paraguay applying molecular techniques
- Species:
- dog
Plain-English summary
A group of dogs in Paraguay was found to have a disease called trypanosomiasis, caused by the parasite Trypanosoma evansi. This is the first time this parasite has been reported in dogs in the country. The researchers used a special testing method called real-time PCR to identify the parasite in the dogs. This technique not only confirmed the presence of T. evansi but also helps differentiate it from other similar parasites. The findings highlight the importance of advanced testing methods in diagnosing diseases in pets.
People also search for: dog parasite infection · Trypanosoma evansi in dogs · trypanosomiasis symptoms in pets
Abstract
Surra is a disease caused by the hemoflagellate pathogen Trypanosoma evansi which affects a wide variety of mammals. The only cases reported of the presence of T. evansi in Paraguay were identified in samples from horses and capybaras and they were detected by light microscopy in the 19th century. The aim of this study is to report three autochthonous cases of canine trypanosomiasis caused by T. evansi, for the first time in the country, using molecular techniques and their application for the differential diagnosis of trypanosomatids species. The technique implemented was real-time PCR-HRM, amplifying a fragment of the hsp70 gene, using a pair of primers initially used to discriminate Leishmania species. This is the first report on the usage of these primers to detect T. evansi through HRM analysis, which allows the differentiation of trypanosomatids species simultaneously, making it an efficient tool for differential diagnosis.
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Search related cases →Original publication on DOAJ: https://doi.org/10.29374/2527-2179.bjvm001920