Peer-reviewed veterinary case report
Footrot on a sheep breeding farm in the Himalayan state of Jammu and Kashmir.
- Journal:
- Revue scientifique et technique (International Office of Epizootics)
- Year:
- 2010
- Authors:
- Taku, A et al.
- Affiliation:
- Faculty of Veterinary Sciences & Animal Husbandry · India
Abstract
In the present study ovine footrot was detected clinically on a sheep farm in the Himalayan state of Jammu and Kashmir. Dichelobacter nodosus was confirmed by culture and polymerase chain reaction (PCR) using species-specific 16S ribosomal RNA primers. When cultured, the organism appeared as flat colourless colonies having a fine granulated structure with irregular margins, and showing characteristic Gram-negative rods with swollen ends. Detection by PCR from cultured bacteria resulted in amplification of a 783 base pairs (bp) product. Serogrouping by multiplex PCR using group (A-I)-specific primers revealed the presence of serogroup B-specific bands of 283 bp.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21309465/