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Peer-reviewed veterinary case report

Molecular detection and characterization of Dichelobacter nodosus in ovine footrot in India.

Journal:
Molecular and cellular probes
Year:
2004
Authors:
Wani, S A et al.
Affiliation:
Faculty of Veterinary Sciences and A.H. · India

Abstract

Dichelobacter nodosus was detected in three clinical cases of ovine footrot in Kashmir, India. The detection was done by PCR in three clinical specimens directly, without isolating the organism, using species-specific 16S rDNA primers. Positive results were indicated by amplification of a 783 bp product. All the three samples were subjected to serogrouping by multiplex PCR using group (A-I) specific primers. All the three samples revealed the presence of serogroup B of D. nodosus by yielding a single band of 283 bps.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/15294315/