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Peer-reviewed veterinary case report

Fortification of α-Lactalbumin and β-Casein in Infant Formula Alleviate Dextran Sulfate Sodium-Induced Colitis in a Young Rat Model: Assessment of Gut Barrier, Inflammatory Response, and Metabolites.

Journal:
Journal of agricultural and food chemistry
Year:
2026
Authors:
Zhao, Ju et al.
Affiliation:
School of Food Science and Technology · China
Species:
rodent

Abstract

This study evaluated &#x3b1;-lactalbumin (&#x3b1;-La)- and &#x3b2;-casein (&#x3b2;-CN)-fortified infant formula (whey:casein 60:40) on intestinal inflammation and metabolism in dextran sulfate sodium (DSS)-induced colitis in young rats. Fortification with &#x3b1;-La and &#x3b2;-CN significantly improved colonic morphology, reduced serum markers of barrier dysfunction, and upregulated epithelial junction proteins, especially occludin (< 0.05). Fortification with &#x3b1;-La and &#x3b2;-CN significantly modulated both proinflammatory and anti-inflammatory cytokine levels in colitis-induced rats. In parallel, &#x3b1;-La and &#x3b2;-CN fortification induced genus-level shifts in gut microbiota composition, characterized by an increased abundance of beneficial genera (e.g.,) and a decrease in potentially proinflammatory taxa (e.g.,). Moreover, &#x3b1;-La and &#x3b2;-CN fortification modulated the levels of several arachidonic acid and glycerophospholipid metabolites. Correlation analysis showed that prostaglandin D(PGD) and 15-deoxy-&#x394;-prostaglandin J(15d-PGJ) were positively associated withand, whereas 2-arachidonoylglycerol (2-AG) was positively correlated withand

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Original publication: https://pubmed.ncbi.nlm.nih.gov/42013210/