Gene Expression as a Potential Aging Biomarker in Dogs.

Abstract

Describing evolutionary conserved physiological or molecular patterns, which can reliably mark the age of both model organisms and humans or predict the onset of age-related pathologies has become a priority in aging research. The age-related gene-expression changes of the() gene have been well-documented in humans and rodents. However, data is lacking from other relevant species, including dogs. Therefore, we quantified themRNA abundance in dogs of different ages, in four tissue types: the frontal cortex of the brain, temporal muscle, skin, and blood. We found a significant, positive correlation betweenrelative expression values and age in the brain, muscle, and blood; however, no correlation was detected in the skin. The strongest correlation was detected in the brain tissue (:= 0.757,< 0.001), similarly to human findings, while the muscle and blood showed weaker, but significant correlation. Our results suggest thatmight be a potential blood-borne biomarker of aging in dogs, although the validation and optimization will require further, more focused research. Our current results also clearly demonstrate that the role ofin aging is conserved in dogs, regarding both tissue specificity and a pivotal role ofin brain aging.