Gene transcription of pro-inflammatory cytokines and chemokines induced by IL-17A in canine keratinocytes.

Abstract

BACKGROUND: Pro-inflammatory cytokines and chemokines produced by activated keratinocytes play an important role in the pathogenesis of canine atopic dermatitis (AD) as well as human AD. Recent studies suggest that keratinocytes activated by IL-17A are involved in the pathogenesis of human AD. However, the role of IL-17A in canine keratinocytes is poorly understood. HYPOTHESIS/OBJECTIVES: Interleukin-17A would induce the transcription of pro-inflammatory cytokines and chemokines in canine keratinocytes. METHODS: The transcription levels of pro-inflammatory cytokines and chemokines were quantified in a canine keratinocyte cell line stimulated with recombinant canine (rc) IL-17A. RESULTS: The transcription of GM-CSF, S100A8, IL-8 and IL-19 in cultured keratinocytes was significantly enhanced at 24 h after stimulation with rcIL-17A. CONCLUSIONS AND CLINICAL IMPORTANCE: Keratinocytes activated by IL-17A have the ability to produce various pro-inflammatory cytokines and chemokines, suggesting that IL-17A may play a central role of the development of Th2-associated inflammation in canine AD.