Peer-reviewed veterinary case report
Generation, characterization and therapeutic potential of anti-feline TNF-alpha MAbs for feline infectious peritonitis.
- Journal:
- Research in veterinary science
- Year:
- 2013
- Authors:
- Doki, Tomoyoshi et al.
- Affiliation:
- School of Veterinary Medicine · Japan
- Species:
- cat
Abstract
Feline infectious peritonitis (FIP) is a lethal infectious disease affecting domestic and wild cats. Several reports suggested that TNF-alpha is related to the progression of FIP. Thus, the administration of a feline TNF-alpha-neutralizing antibody to cats with FIP may reduce the disease progression. In this study, we have prepared nine monoclonal antibodies (MAbs) that recognize feline TNF-alpha. All MAbs neutralized recombinant TNF-alpha. The 50% inhibitory concentrations (IC50) of the MAbs for the cytotoxicity of recombinant TNF-alpha were 5-684 ng/ml. MAb 2-4 exhibited high neutralizing activity against natural TNF-alpha derived from FIPV-infected macrophages, and was confirmed to inhibit the following feline TNF-alpha-induced conditions in vitro: (i) an increase in the survival rate of neutrophils from cats with FIP, (ii) aminopeptidase N (APN) mRNA expression in macrophages, and (iii) apoptosis of a feline T-lymphocyte cell line.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24095161/