Peer-reviewed veterinary case report
New antibody developed to treat lymphoma in dogs
By Mizuno, Takuya et al.·Published in Scientific Reports·2020·View original on Crossref →
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Original publication title: Generation of a canine anti-canine CD20 antibody for canine lymphoma treatment
- Species:
- dog
Plain-English summary
A new treatment for dogs with lymphoma, a common type of cancer, has been developed using a special antibody designed specifically for canines. Researchers created a chimeric antibody that targets B cells, which are often involved in this cancer. In tests, this antibody showed strong effectiveness in killing lymphoma cells and even led to significant tumor growth suppression in a mouse model. Additionally, when given to healthy beagles, it effectively reduced B cells in their blood, indicating it could be a promising option for treating dogs with B cell lymphoma.
People also search for: dog lymphoma treatment · canine cancer antibody · beagle lymphoma symptoms
Abstract
AbstractLymphoma is the most common hematological cancer in dogs. Canine diffuse large B cell lymphoma shows a relatively good response to treatment with multi-agent cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) chemotherapy; however, the 2-year survival rate is as low as 20%. For human B cell type lymphoma, the anti-CD20 chimeric antibody, rituximab, was developed two decades ago. The combination of rituximab and CHOP chemotherapy was highly successful in improving patient prognosis. However, no anti-canine CD20 antibody is available for the treatment of canine lymphoma. During this study, a rat anti-canine CD20 monoclonal antibody was established. We also generated a rat-canine chimeric antibody against canine CD20 designed for clinical application. This chimeric antibody (4E1-7-B) showed in vitro antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) against the canine B cell lymphoma cell line CLBL-1. Moreover, to obtain stronger ADCC activity, a defucosylated 4E1-7-B antibody (4E1-7-B_f) was also generated, and it showed tenfold stronger ADCC activity compared with 4E1-7-B. 4E1-7-B_f as well as 4E1-7-B suppressed the growth of CLBL-1 tumors in an immunodeficient xenotransplant mouse model. Finally, a single administration of 4E1-7-B_f induced considerable peripheral B cell depletion in healthy beagles. Thus, 4E1-7-B_f is a good antibody drug candidate for canine B cell type lymphoma.
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Search related cases →Original publication on Crossref: https://doi.org/10.1038/s41598-020-68470-9