Peer-reviewed veterinary case report
Best antigens found for diagnosing leishmaniosis in dogs
By Rami, M et al.·Published in Veterinary parasitology·2005·Department of Parasitology and Parasitic Diseases·View original on PubMed →
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Original publication title: Identification of two highly performing Leishmania infantum antigens for serodiagnosis of canine leishmaniosis.
- Species:
- dog
Plain-English summary
A study found two specific proteins from the Leishmania infantum parasite that can help diagnose leishmaniosis in dogs. Researchers tested blood samples from 31 dogs confirmed to have the disease and identified proteins that triggered immune responses. The two proteins, weighing 14 and 16 kDa, were consistently recognized in all infected dogs and could be detected even in very diluted serum samples. This means these proteins could be useful for more accurate testing in dogs suspected of having leishmaniosis, reducing the chances of false positives seen with other tests.
People also search for: dog leishmaniosis symptoms · canine leishmaniosis diagnosis · Leishmania test for dogs
Abstract
In the aim of improving serodiagnosis of canine leishmaniosis, we analysed the humoral immune response of dog against Leishmania infantum parasite. The antigenic reaction of L. infantum polypeptides with sera from 31 dogs with parasitologically confirmed leishmaniosis was studied by using the immunoblot technique. Electrophoretic profile of the parasite extract showed more than 50 polypeptides, with molecular weights ranging from 12 to 170 kDa. Among these polypeptides, 37 antigen components, ranging from 14 to 91 kDa, were recognised by antibodies of L. infantum infected dogs. Three polypeptides (14, 16 and 76 kDa) reacted with all of the 31 serum samples. The other most frequently recognised antigens were those of 29.5, 32, 46, 59 and 66 kDa with a sensitivity of 87.1%, 93.6%, 96.8%, 87.1% and 80.6%, respectively. The 14 and 16 kDa bands were the most intense and remained detectable until a serum dilution of 1:6400. No reaction of these two major antigens was observed with sera collected from 50 Leishmania-free dogs, living in the leishmaniosis-free region of Rabat in Morocco, whereas the crude antigen used in IFAT or ELISA lead to three false positive results. Four antigen components of 29, 41, 55, and 70 kDa were recognised by some sera samples from negative controls. These results demonstrated the potential interest of the fractions of 14 and 16 kDa in immunodiagnosis of canine leishmaniosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/16076527/