Immunocytochemical detection of B cells in the guinea pig pancreas using guinea pig antiserum to porcine insulin.

Abstract

The purpose of this study was to determine if antisera to an heterologous insulin could replace specific anti-guinea pig insulin serum for immunoperoxidase localization of guinea pig B cells. We found that antisera to porcine insulin was effective for this purpose even though it was produced by guinea pigs. The immunostaining results obtained with anti-porcine insulin sera and anti-guinea pig insulin serum were identical, by both light and electron microscopic immunoperoxidase staining. Control experiments supported the conclusion that the guinea pig antisera to porcine insulin contained antibodies that reacted with guinea pig insulin.