Peer-reviewed veterinary case report
Tumor protein markers found in bone cancer of 145 dogs
By Russell, Duncan S et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2018·Department of Biomedical Sciences·View original on PubMed →
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Original publication title: Immunohistochemical detection of p53, PTEN, Rb, and p16 in canine osteosarcoma using tissue microarray.
- Species:
- dog
Plain-English summary
A group of dogs with osteosarcoma, a type of bone cancer, were studied to understand the presence of certain proteins that help control cell growth. The dogs, mostly purebreds like Greyhounds and Rottweilers, were around 7 years old on average. Researchers found that proteins p53, PTEN, Rb, and p16 were present in varying amounts in the tumors, but their levels did not significantly affect how long the dogs lived after diagnosis. This study helps to better understand the biology of osteosarcoma in dogs, but it didn't find a direct link between these proteins and survival outcomes.
People also search for: dog osteosarcoma treatment · Greyhound bone cancer symptoms · Rottweiler cancer survival rate
Abstract
Although inactivating mutations of tumor suppressor genes are well described in cell lines of canine osteosarcoma (OS), expression of tumor suppressor proteins in spontaneous disease is poorly characterized. We determined the immunohistochemical expression of p53, PTEN, Rb, and p16 in a large cohort of dogs with OS. Formalin-fixed, paraffin-embedded samples of canine OS were analyzed retrospectively. Primary tumor samples from 145 dogs, collected between 2003 and 2008, were evaluated by tissue microarray. Streptavidin-biotin complex immunohistochemistry was performed using monoclonal antibodies for Rb and PTEN and polyclonal antibodies for p16 and p53. The average age of dogs was 7.6 y, and 118 of 145 (81%) were purebred. Most commonly represented purebreds were Greyhound (23%), Rottweiler (11%), and Labrador Retriever (10%). Immunohistochemical detection of p53, PTEN, Rb, and p16 was 81%, 61%, 66%, and 66%, respectively. The staining pattern for p16 was primarily cytoplasmic; the predominant pattern for PTEN, Rb, and p53 was cytoplasmic and nuclear. Exclusively cytoplasmic staining was noted in 19% of samples positive for p53 and 8% of samples positive for Rb. Kaplan-Meier curves showed that protein expression was not associated with significant differences in overall survival ( p > 0.191). We documented heterogeneity in both immunostaining and subcellular localization of tumor suppressor proteins, providing further characterization of canine OS.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/29629647/