Peer-reviewed veterinary case report
In situ hybridization with labeled probes: assessment of african Swine Fever virus in formalin-fixed paraffin-embedded tissues.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2015
- Authors:
- Ballester, Maria & Rodríguez, Fernando
- Affiliation:
- Centre de Recerca en Sanitat Animal (CReSA) · Spain
Abstract
In situ hybridization (ISH) has become a very valuable molecular diagnostic tool to detect specific DNA or RNA sequences in biological samples through the use of complementary DNA- or RNA-labeled probes. Here, we describe an optimized in situ hybridization protocol to detect African swine fever virus (ASFV) DNA in formalin-fixed, paraffin-embedded tissues using digoxigenin-labeled probes.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25399099/