In vitroeffects ofYunnan Baiyaoon canine hemangiosarcoma cell lines

Abstract

AbstractYunnan Baiyaois a Chinese herbal medicine that has been utilized for its anti‐inflammatory, haemostatic, wound healing and pain relieving properties in people. It has been utilized in the veterinary profession to control bleeding in dogs with hemangiosarcoma (HSA) and has been anecdotally reported to prolong survival times in dogs with this neoplasm. This study evaluated thein vitroactivity ofYunnan Baiyaoagainst three canine HSA cell lines after treatment with increasing concentrations ofYunnan Baiyao(50, 100, 200, 400, 600 and 800 µg mL−1) at 24, 48 and 72 h. Mean half maximum inhibitory concentration (IC50) at 72 h for DEN, Fitz, SB was 369.9, 275.9 and 325.3 µg mL−1, respectively. Caspase‐3/7 activity increased in correlation with the IC50in each cell line which was confirmed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, APO‐BRDU Kit; BD Biosciences, San Jose, CA, USA) assay. VEGF in cell supernatant was also quantified. Overall, the study found thatYunnan Baiyaocauses dose and time dependent HSA cell death through initiation of caspase‐mediated apoptosis, which supports future studies involvingYunnan Baiyao.