Investigation of a relationship between serum concentrations of microRNA-122 and alanine aminotransferase activity in hospitalised cats.

Abstract

OBJECTIVES: Current blood tests to diagnose feline liver diseases are suboptimal. Serum concentrations of microRNA (miR)-122 have been shown in humans, dogs and rodents to be a sensitive and specific biomarker for liver injury. To explore the potential diagnostic utility of measuring serum concentrations of miR-122 in cats, miR-122 was measured in a cohort of ill, hospitalised cats with known serum alanine aminotransferase (ALT) activity. METHODS: In this retrospective study, cats were grouped into those with an ALT activity within the reference interval (0-83&#x2009;U/l; n&#x2009;=&#x2009;38) and those with an abnormal ALT activity (>84&#x2009;U/l; n&#x2009;=&#x2009;25). Serum concentrations of miR-122 were measured by real-time quantitative PCR and the relationship between miR-122 and ALT was examined. RESULTS: miR-122 was significantly higher in the group with high ALT activity than the ALT group, within normal reference limits (<0.0004). There was also a moderately positive correlation between serum ALT activity and miR-122 concentrations (<0.001;&#x2009;=&#x2009;0.52). CONCLUSIONS AND RELEVANCE: Concentrations of miR-122 were reliably quantified in feline serum and were higher in a cohort of cats with increased ALT activity than in cats with normal ALT activity. This work highlights the potential diagnostic utility of miR-122 as a biomarker of liver damage in cats and encourages further investigation to determine the sensitivity and specificity of miR-122 as a biomarker of hepatocellular injury in this species.