Peer-reviewed veterinary case report
Ehrlichia canis strain from southeastern Brazil isolated and studied
By Alves, Rosiane Nascimento et al.·Published in Journal of veterinary science·2014·Institute of Biomedical Sciences, Brazil·View original on PubMed →
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Original publication title: Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil.
- Species:
- dog
Plain-English summary
A dog in southeastern Brazil tested positive for Ehrlichia canis, a type of bacteria that can cause serious health issues. Blood samples were taken to confirm the infection, and researchers isolated a new strain called Uberlandia. They found that this strain had similar genetic markers to other strains worldwide, indicating it is closely related. Tests showed that the antigens from this strain could effectively identify other dogs infected with Ehrlichia canis in Brazil. This research helps improve understanding and diagnosis of this infection in dogs.
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Abstract
Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlandia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28 partial sequence for the E. canis Uberlândia strain differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlândia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlândia and São Paulo). The results suggest that both antigens were able to identify animals seropositive for E. canis in Brazil since these Brazilian strains appear to be highly conserved.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24136211/