Peer-reviewed veterinary case report
Antibodies slowing Leishmania growth in dogs with visceral
By Maia, Ana Carolina Ribeiro Gomes et al.·Published in Veterinary parasitology·2019·Departamento de Bioquí, Brazil·View original on PubMed →
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Original publication title: Leishmania infantum nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) B-domain: Antibody antiproliferative effect on the promastigotes and IgG subclass responses in canine visceral leishmaniasis.
- Species:
- dog
Plain-English summary
A study found that a specific protein on the surface of Leishmania infantum, the parasite that causes visceral leishmaniasis in dogs, can trigger an immune response. Researchers tested antibodies against this protein and discovered they could significantly reduce the growth of the parasite in lab conditions. The findings suggest that these antibodies could help protect dogs from infection and may lead to new treatments or vaccines. While this research is promising, further studies are needed to see how these findings can be applied in real-world situations for dogs suffering from leishmaniasis.
People also search for: dog leishmaniasis treatment · canine leishmaniasis vaccine · symptoms of leishmaniasis in dogs
Abstract
A nucleoside triphosphate diphosphohydrolase-1 (NTPDase 1) was identified on the surface, flagellum and kinetoplast from L. infantum promastigotes by immunocytochemistry and confocal laser scanning microscopy, using immune sera that recognized specifically the B domain of NTPDase 1 and produced against synthetic peptides (LbB1LJ and LbB2LJ) derived from this domain. The polyclonal antibodies had effective antileishmanial effect, reducing significantly in vitro promastigotes growth (21-25%), an antiproliferative effect also demonstrated by immune sera produced against recombinant r-pot B domain, and two other synthetic peptides (potB1LJ and potB2LJ). In addition, using these biomolecules in ELISA technique, IgG1 and IgG2 subclasses reactivities of either healthy dogs or infected by L. infantum and classified clinically as asymptomatic, oligosymptomatic and symptomatic were tested. Analysis of distinct IgG1 and IgG2 seropositivities patterns suggested antibody subclasses binding epitopes along B domain for protection against infection, indicating this domain as a new tool for prophylactic and immunotherapeutic investigations.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31303201/