Peer-reviewed veterinary case report
Comparing liquid-based and standard cytology for diagnosing lymphoma
By Fernandes, N C C A et al.·Published in Veterinary and comparative oncology·2016·Centro de Patologia·View original on PubMed →
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Original publication title: Liquid-based cytology and cell block immunocytochemistry in veterinary medicine: comparison with standard cytology for the evaluation of canine lymphoid samples.
- Species:
- dog
Plain-English summary
A group of 18 dogs with swollen lymph nodes were tested for lymphoma using a new method called liquid-based cytology (LBC) alongside traditional cytology techniques. The LBC method provided clearer cell details, which helped in identifying the type of cells present. The study found that combining LBC with a specific cell block technique improved the accuracy of lymphoma diagnosis. This approach could help veterinarians better diagnose and treat dogs with lymphoma in the future.
People also search for: dog swollen lymph nodes lymphoma diagnosis · canine lymphoma treatment options · liquid-based cytology for dogs
Abstract
Liquid-based Cytology (LBC) consists of immediate wet cell fixation with automated slide preparation. We applied LBC, cell block (CB) and immunocytochemistry to diagnose canine lymphoma and compare results with conventional cytology. Samples from enlarged lymph nodes of 18 dogs were collected and fixed in preservative solution for automated slide preparation (LBC), CB inclusion and immunophenotyping. Two CB techniques were tested: fixed sediment method (FSM) and agar method (AM). Anti-CD79a, anti-Pax5, anti-CD3 and anti-Ki67 were used in immunocytochemistry. LBC smears showed better nuclear and nucleolar definition, without cell superposition, but presented smaller cell size and worse cytoplasmic definition. FSM showed consistent cellular groups and were employed for immunocytochemistry, while AM CBs presented sparse groups of lymphocytes, with compromised analysis. Anti-Pax-5 allowed B-cell identification, both in reactive and neoplastic lymph nodes. Our preliminary report suggests that LBC and FSM together may be promising tools to improve lymphoma diagnosis through fine-needle aspiration.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25665030/