Peer-reviewed veterinary case report
Methotrexate causes high cancer cell death in canine lymphoma
By Pawlak, Aleksandra et al.·Published in Research in veterinary science·2017·Department of Pharmacology and Toxicology·View original on PubMed →
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Original publication title: Methotrexate induces high level of apoptosis in canine lymphoma/leukemia cell lines.
- Species:
- dog
Plain-English summary
A study found that methotrexate, a chemotherapy drug, can effectively kill cancer cells in dogs with lymphoma and leukemia. The researchers tested different dog cancer cell lines and discovered that methotrexate was particularly effective against a type of T cell cancer that usually resists other treatments. This suggests that methotrexate could be a valuable option for treating these types of cancers in dogs. While this research is still in the early stages, it shows promise for improving cancer treatment in pets.
People also search for: dog lymphoma treatment · methotrexate for dog cancer · canine leukemia chemotherapy
Abstract
INTRODUCTION: Methotrexate is an antimetabolite used in the treatment of cancer and non-malignant diseases including rheumatoid arthritis, psoriasis and graft vs. host disease. Combination therapy with methotrexate was successful in the treatment of canine lymphoma, mammary tumor and invasive urinary bladder cancer. Lymphoma, the most common hematopoietic cancer in dogs, and leukemia are sensitive to chemotherapy, which is why methotrexate may be an important treatment option for these diseases. Although methotrexate is already used in veterinary oncology its effects on canine cancer cells has not been tested. The aim of the study was to evaluate for the first time methotrexate concentration-dependent cytotoxicity and its capability of inducing apoptosis in selected canine lymphoma/leukemia cell lines: CLBL-1, GL-1 and CL-1 as a first step before the in vitro development of new therapeutic options with the use of methotrexate. RESULTS: Methotrexate exhibited concentration-dependent inhibitory effect on proliferation of all the examined cell lines with different degree of apoptosis induction. The most methotrexate sensitive cells belonged to CL-1 cell line derived from T cell neoplasia and previously characterized by high resistance to the majority of anticancer drugs used in the therapy of lymphoma/leukemia in dogs. Canine lymphoma and leukemia cell lines are sensitive to methotrexate, and this drug may be useful in effective treatment of canine neoplasms and especially of T-type leukemia/lymphoma.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/28992489/