Peer-reviewed veterinary case report
Detecting Leishmania infantum DNA in dogs at different disease stages
By Fernandes, Murilo Antônio et al.·Published in Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria·2019·Laborató·View original on PubMed →
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Original publication title: Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog.
- Species:
- dog
Plain-English summary
A study found that a group of dogs with leishmaniasis, a disease caused by the Leishmania parasite, showed varying levels of infection based on their clinical symptoms. Out of 215 dogs tested, most had mild disease, while a few had moderate to severe symptoms. The researchers used different blood tests to detect the parasite, and one specific test (13A/13B) was much better at identifying infected dogs, especially those in the early stages of the disease. This suggests that using the right test is crucial for diagnosing leishmaniasis in dogs effectively.
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Abstract
The aim of this study was to compare molecular tests used to diagnose Leishmania spp. in dogs with different stages of infection. Blood and conjunctival swab (CS) samples from dogs classified in four clinical stages were subjected to different PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S and LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) of dogs were classified as without clinical signs, 67.5% (145/215) stage I (mild disease), 7.0% (15/215) stage II (moderate disease) and 3.2% (7/215) stage III (severe disease). The results showed that in blood samples, 13A/13B detected a significant higher number of positive dogs in stage I (25/145) and in total (42/215) (p≤0.05). However, when CS samples were tested, no difference was observed (p>0.05). On the other hand, in blood samples, MC1/MC2 detected significantly fewer positive dogs classified as without clinical signs (0/48), in stage I (0/145) and in total (1/215) (p≤0.05). Likewise, in CS samples, this primers showed also lower detection (1/215) (p≤0.05). So than, we can conclude that PCR on blood samples with 13A/13B primers has greater capacity to detect positive dogs, mainly at the initial of clinical disease than do other primers and MC1/MC2 are not a good choice to detect Leishmania infantum infection in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31188942/