Peer-reviewed veterinary case report
New blood test spots canine visceral leishmaniasis using 12 proteins
By de Oliveira, Isaac Queiroz et al.·Published in Parasites & vectors·2015·Laborató, Brazil·View original on PubMed →
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Original publication title: Multi-antigen print immunoassay (MAPIA)-based evaluation of novel recombinant Leishmania infantum antigens for the serodiagnosis of canine visceral leishmaniasis.
- Species:
- dog
Plain-English summary
A study found that a new testing method could help detect Leishmania infantum infection in dogs, which causes visceral leishmaniasis (VL). This disease can be serious, and dogs are often the main carriers. Researchers tested a combination of proteins to see how well they could identify infected dogs, achieving an 81% detection rate when using five specific proteins. This new method could lead to more accurate and quicker tests for diagnosing VL in dogs, helping veterinarians provide better care for affected pets.
People also search for: dog leishmaniasis symptoms · how to test for leishmania in dogs · canine visceral leishmaniasis treatment
Abstract
BACKGROUND: Domestic dogs are the principal reservoir hosts of Leishmania infantum in regions where visceral leishmaniasis (VL) is endemic. Although serologic methods are frequently used for the screening of infected dogs, antibody-based tests require further assessment, due to lack of sensitivity and specificity. In this study, we employed a multi-antigen printing immunoassay (MAPIA) to compare the antibody responses to novel recombinant proteins of L. infantum with the potential for the detection of canine VL. FINDINGS: MAPIA strips were prepared employing 12 recombinant proteins. Antibody reactivity to these antigens was compared using a panel of sera collected from clinically asymptomatic (n = 16) and symptomatic (n = 41) culture-positive animals. Our findings showed that the canine immune response to antigen differs between dogs and depends on infection status. Using this screening assay, when five out of the 12 antigens were combined, an overall 81% detection rate of L. infantum-infected dogs was achieved. CONCLUSIONS: We conclude that MAPIA is an effective screening tool to rapidly select multiple antigens of diagnostic utility to be used in a more sensitive point of care diagnostic test such as the Dual-Path Platform (DPP) multiplex test for the rapid detection of infected dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25616448/