Peer-reviewed veterinary case report
Fast PCR tests to detect multiple dog respiratory and intestinal
By Xiangqi Hao et al.·Published in PLoS ONE·2019·View original on DOAJ →
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Original publication title: Multiplex PCR methods for detection of several viruses associated with canine respiratory and enteric diseases.
- Species:
- dog
Plain-English summary
A group of dogs showing signs of respiratory or intestinal illness had nasal and anal swabs taken to test for multiple viruses at once. Researchers developed a new test that could quickly identify seven different viruses, including those causing kennel cough and parvovirus. This rapid testing method helps veterinarians diagnose infections more effectively and determine if a dog has more than one virus at the same time. By using this new tool, vets can provide timely treatment to improve the health of affected dogs.
People also search for: dog respiratory infection treatment · dog parvovirus symptoms · how to test for kennel cough in dogs
Abstract
Viral respiratory and intestinal infections are the most common causes of canine viral illness. Infection with multiple pathogens occurs in many cases. Rapid diagnosis of these multiple infections is important for providing timely and effective treatment. To improve diagnosis, in this study, two new multiplex polymerase chain reactions (mPCRs) were developed for simultaneous detection of canine respiratory viruses (CRV) and canine enteric viruses (CEV) using two separate primer mixes. The viruses included canine adenovirus type 2 (CAV-2), canine distemper virus (CDV), canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine circovirus (CanineCV), canine coronavirus (CCoV) and canine parvovirus (CPV). The sensitivity of the mPCR results showed that the detection limit of both mPCR methods was 1×104 viral copies. Twenty nasal swabs (NS) and 20 anal swabs (AS) collected from dogs with symptoms of respiratory disease or enteric disease were evaluated using the novel mPCR methods as a clinical test. The mPCR protocols, when applied to these respiratory specimens and intestinal samples, could detect 7 viruses simultaneously, allowing rapid investigation of CRV (CAV-2, CDV, CIV and CPIV) and CEV (CAV-2, CanineCV, CCoV and CPV) status and prompt evaluation of coinfection. Our study provides an effective and accurate tool for rapid differential diagnosis and epidemiological surveillance in dogs.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1371/journal.pone.0213295