Peer-reviewed veterinary case report
Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness.
- Journal:
- Proteomics
- Year:
- 2004
- Authors:
- Ge, Yue et al.
- Affiliation:
- Department of Proteomics · United States
- Species:
- rodent
Abstract
The Multiplexed Proteomics (MP) technology is a new approach that permits quantitative, multicolor fluorescence detection of proteins in one-dimensional or two-dimensional gels. This methodology allows for multiplexed identification and differential analysis of phosphoproteins, glycoproteins, and total proteins within a single gel electrophoresis experiment. Here the MP system was applied to the differential proteomic analysis of pregnancy-induced refractoriness to breast cancer using a rat model system. Differential analyses identified multiple proteins with altered phosphorylation, glycosylation, or protein expression patterns.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15378738/