Peer-reviewed veterinary case report
Needle rinse cell blocks as an ancillary technique: Diagnostic and clinical utility in gastrointestinal neoplasia.
- Journal:
- Veterinary clinical pathology
- Year:
- 2022
- Authors:
- Marrinhas, Carla et al.
- Affiliation:
- Institute of Biomedical Sciences Abel Salazar
Plain-English summary
This study looked at a technique called needle rinse cell blocks (NRCB) to help diagnose certain types of tumors in the intestines of dogs and cats. Researchers used this method after taking samples from two dogs and one cat with intestinal lymphomas (a type of cancer) and a dog with a gastrointestinal stromal tumor (GIST). They found that the NRCB technique worked well, providing clear results that matched the traditional tissue biopsies. This method is easy to use and not very invasive, making it a helpful tool for veterinarians to get a better understanding of gastrointestinal tumors. Overall, the treatment was effective in confirming the diagnoses.
Abstract
BACKGROUND: Fine-needle aspirate (FNA) cytology is often the first-choice method for diagnosing gastrointestinal nodular lesions. The FNA material can be converted to histopathology specimens by a needle rinse cell block (NRCB) technique, allowing ancillary studies to refine the cytologic diagnosis. Despite use in human pathology, NRCB has never been applied to canine or feline gastrointestinal neoplasia. OBJECTIVE: This study described NRCB methodology and its diagnostic utility in specific cases of neoplastic gastrointestinal lesions. METHODS: Needle rinses with saline were performed after ultrasound-guided FNAs of two intestinal lymphomas (canine and feline) and a canine gastrointestinal stromal tumor (GIST). The NRCB was prepared using the cell tube block technique and processed for paraffin embedding. Routine immunohistochemistry protocols (using CD3, PAX-5, and Ki-67 for lymphoma cases and vimentin, desmin, S-100, and KIT markers for GIST) were applied to NRCB sections, and the results were compared with matched tissue biopsies. RESULTS: NRCBs with adequate cell numbers, preservation, and good separation of blood were obtained. The diagnosis and immunophenotyping were confirmed in both cases of lymphoma in NRCBs. In the GIST, the immunolabeling of the neoplastic cells in NRCB was completely concordant with the tissue biopsy. CONCLUSIONS: The described methodology is suitable for veterinary settings, having few technical requirements and low invasiveness. The presented cases of gastrointestinal neoplasia highlight the utility of NRCBs as a platform to conduct ancillary studies and refine the cytologic diagnosis.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/34642969/