Occurrence and Multi-Locus Genotyping ofin Black Goats from Fujian Province, China.

Abstract

is an important zoonotic intestinal protozoon causing diarrhea in humans and animals, threatening public health and livestock farming. This study aimed to investigate the infection and genotype distribution ofin black goats in Fujian, southeastern China. A total of 539 fecal samples were collected from nine regions.was detected by nested PCR and sequencing targeting the ITS locus, and genotyped using four microsatellites and one minisatellite. The overall infection rate was 7.79%, with 42 positive samples. Eight genotypes were identified, including seven known genotypes and one novel genotype FJG-1. Ten multilocus genotypes (MLGs) were obtained, and all isolates belonged to Group 2. The infection rate differed significantly among regions (< 0.01), but not among ages or genders (> 0.05). This is the first molecular epidemiological survey ofin black goats in Fujian. The results enrich the genotype database and provide data for formulating regional prevention and control strategies against this pathogen.