Occurrence and Multilocus Genotyping ofin Yunnan Black Goats in China.

Abstract

is an important zoonotic parasite which can parasitize in the intestines of humans and various animals. However, the information about the prevalence and genetic diversity ofin goats in China is limited. It is yet to be known whether Yunnan black goats, a unique goat breed in subtropical Yunnan province, southwestern China, are infected withThus, a total of 907 fecal samples were collected from Yunnan black goats in five regions in Yunnan province, to estimate the prevalence and genotypes ofusing a PCR-based approach. Theprevalence is 4.2% (38/907) in Yunnan black goats by nested amplification of the-giardin (bg) gene, and the genotypes are identified as assemblage E, with 5 novel subtypes (E-E). Multilocus sequence typing revealed that 11, 18, and 38 samples were amplifiable on tpi (triose phosphate isomerase), gdh (glutamate dehydrogenase), and bg locus, respectively, and identified three novel multilocus genotypes (MLGs): MLGE9-MLGE11. To our knowledge, this is the first report ofprevalence and genotypes in Yunnan black goats in China, which extended the host range ofand provided basic data for controllinginfection in Yunnan black goats.