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Peer-reviewed veterinary case report

PCR test accuracy for Leishmania and Ehrlichia in Brazilian dogs

By Silveira, Ana Paula Stefanello da et al.·Published in Revista da Sociedade Brasileira de Medicina Tropical·2018·Programa de P&#xf3·View original on PubMed

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Original publication title: PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil.

Species:
dog

Plain-English summary

A group of 400 dogs in Brazil showed signs of illness due to infections with Leishmania and Ehrlichia. Most of these dogs had multiple symptoms, and tests revealed that a significant number were positive for Leishmania. The study found that using a specific blood test (PCR) can help diagnose Leishmania infections, especially when other tests are inconclusive. This information can be crucial for veterinarians in identifying and treating these infections effectively.

People also search for: dog Leishmania symptoms · Ehrlichia infection in dogs · PCR test for dog blood infection

Abstract

INTRODUCTION: Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized. METHODS: PCR and parasitological tests were conducted. RESULTS: PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples. CONCLUSIONS: Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/30517541/