Peer-reviewed veterinary case report
Testing a new local antigen for detecting canine visceral
By Semião-Santos, Saul José et al.·Published in Journal of medical microbiology·2014·Instituto de Ciê·View original on PubMed →
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Original publication title: Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis.
- Species:
- dog
Plain-English summary
A group of dogs suspected of having canine visceral leishmaniasis (CVL) were tested using a new locally made antigen and a standard reference antigen to see which was more effective. The new test showed a perfect sensitivity and specificity, meaning it accurately identified dogs with and without the disease. The researchers found that the new antigen was easier to use and read, suggesting it could be a reliable option for confirming or ruling out CVL in dogs.
People also search for: dog leishmaniasis test · symptoms of canine visceral leishmaniasis · how to test for leishmaniasis in dogs
Abstract
We compared the performance of a locally produced β-mercaptoethanol-modified promastigote antigen (β-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for β-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for β-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen's κ = 0.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of β-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24143006/