Peer-reviewed veterinary case report
New needle method for testing dog lymph nodes for visceral
By Artur Augusto Velho Mendes Júnior et al.·Published in Animals·2025·Carlos Chagas Institute, Oswaldo Cruz Foundation, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba 81350-010, Brazil, CH·View original on DOAJ →
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Original publication title: Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis
- Species:
- dog
Plain-English summary
A group of dogs with a serious infection called visceral leishmaniasis (caused by the parasite Leishmania) had samples taken from their lymph nodes to help diagnose the disease. Researchers tested a new method using a semi-automatic needle to collect these samples and compared it to traditional methods. They found that the new method was effective, with a high success rate in detecting the parasite, and it was quicker and easier than the other techniques. This could be a promising option for vets when diagnosing this disease in dogs.
People also search for: dog leishmaniasis symptoms · lymph node biopsy in dogs · diagnosing leishmania in dogs
Abstract
Zoonotic visceral leishmaniasis is caused by <i>Leishmania</i> (<i>Leishmania</i>) <i>infantum</i> and dogs are the main domestic reservoir. This study compared the performance of parasitological tests using semi-automatic needle puncture (SANP) for collecting popliteal lymph node samples with samples collected from the same lymph node by fine needle aspiration puncture (FNAP) and by necropsy for the diagnosis of canine visceral leishmaniasis (CVL). Popliteal lymph node samples were collected from 30 CVL-seropositive dogs from an endemic region in Brazil. After clinical examination and euthanasia, samples were collected from the same lymph node by SANP, FNAP, and necropsy. The reference tests were culture, immunohistochemistry, and histopathology. Positivity for <i>Leishmania</i> spp. was 70% for immunohistochemistry and 33.3% for histopathology. Culture positivity using the different sampling techniques was 77% for necropsy (87% in the first week), 73% for FNAP (82% in the first week), and 63% for SANP (95% in the first week). The combination of SANP and culture proved to be an alternative for the diagnosis of <i>Leishmania</i> spp. in the lymph node samples of dogs because of its high positivity rate and because it is more practical and faster and has a shorter time to positivity by culture when compared to FNAP and necropsy sampling.
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Search related cases →Original publication on DOAJ: https://doi.org/10.3390/ani15010107