Peer-reviewed veterinary case report
Accuracy of rK39 ELISA test for diagnosing dog visceral leishmaniasis
By Fernanda Alvarenga Cardoso Medeiros et al.·Published in Cadernos de Saúde Pública·2021·View original on DOAJ →
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Original publication title: Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil
- Species:
- dog
Plain-English summary
A study tested a new blood test for diagnosing visceral leishmaniasis in dogs, which is a serious disease spread by parasites. The test, called ELISA-Vetlisa/BIOCLIN, was evaluated using samples from 200 dogs, including those showing symptoms and healthy ones. It showed an impressive 99% sensitivity and 100% specificity, meaning it accurately identified dogs with the disease without false positives. This test could be a reliable option for veterinarians in Brazil to diagnose this condition effectively.
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Abstract
Abstract: Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISA-Vetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1590/0102-311x00041320