Peer-reviewed veterinary case report
Accuracy of rK39 ELISA test for diagnosing dog leishmaniasis in Brazil
By Medeiros, Fernanda Alvarenga Cardoso et al.·Published in Cadernos de saude publica·2021·Fundaç·View original on PubMed →
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Original publication title: Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil.
- Species:
- dog
Plain-English summary
A study in Brazil tested a new blood test called ELISA-Vetlisa/BIOCLIN to diagnose visceral leishmaniasis in dogs, a serious disease that can be transmitted to humans. The test was performed on samples from 200 dogs, including those showing symptoms and healthy ones. The results showed that the test was highly accurate, correctly identifying nearly all infected dogs and not giving false positives. This new test could help veterinarians diagnose this disease more effectively, leading to better treatment and control measures for affected dogs.
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Abstract
Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISA-Vetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/33852659/