Peer-reviewed veterinary case report
Using plasma cell-free DNA to detect lymphoma in dogs
By Kambayashi, Satoshi et al.·Published in Veterinary and comparative oncology·2024·Department of Veterinary Internal Medicine, Japan·View original on PubMed →
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Original publication title: Plasma cell-free DNA in canine lymphoma patients as a novel material for genotyping.
- Species:
- dog
Plain-English summary
A group of dogs with lymphoma, a type of cancer that affects the immune system, had their blood tested for cell-free DNA (cfDNA) levels. The study found that dogs with lymphoma had much higher levels of cfDNA compared to healthy dogs, suggesting that this could be a useful marker for diagnosing the disease. Additionally, the researchers used cfDNA to analyze the genetic makeup of the lymphomas and found a strong correlation with results from traditional tissue samples. This means that measuring cfDNA in the blood could help vets diagnose lymphoma more accurately and monitor the disease.
People also search for: dog lymphoma symptoms · canine cancer blood test · lymphoma treatment for dogs
Abstract
Canine lymphoma is a disease with high morbidity and poor long-term prognosis, despite a high response rate to chemotherapy. In this study, we focused on liquid biopsy, in which small amounts of substances from body fluids were analysed, to determine whether cell-free DNA (cfDNA) in the plasma can be used as a biomarker for lymphoma in dogs. We found that 23 patients with lymphoma had significantly higher cfDNA concentrations than the 12 healthy dogs (median 2360 ng/mL versus 299 ng/mL, p < .0001). Polymerase chain reaction for antigen receptor rearrangement (PARR) was also employed using cfDNA from the lymphoma group to investigate whether cfDNA could be used for the detection of genetic clonality of lymphomas, as well as the genomic DNA (gDNA) extracted from an original lesion in each case. The correlation of the PARR results between cfDNA and gDNA was observed in 100% of B-cell lymphomas (10/10), 77.8% of T-cell lymphomas (7/9), and 100% of other types of lymphomas (4/4), respectively. These results indicate that plasma cfDNA levels are increasing in canine lymphoma patients, that cfDNA concentration can be a novel diagnostic tool, and that it can be used as a diagnostic tool for PARR.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38287199/