Peer-reviewed veterinary case report
Detecting platelet antibodies in dogs with immune thrombocytopenia
By Brooks, Marjory B. et al.·Published in Veterinary Clinical Pathology·2022·Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine Cornell University Ithaca NY USA, United States·View original on Crossref →
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Original publication title: Preliminary evaluation of a flow cytometric assay with microsphere controls for the detection of platelet‐bound antibodies in canine immune thrombocytopenia
- Species:
- dog
Plain-English summary
A group of dogs with immune thrombocytopenia (ITP), a condition that can cause severe bleeding, were tested to see if a new method could help detect antibodies that might worsen their condition. Researchers developed a special test using microspheres coated with dog antibodies to improve the accuracy of results. They found that dogs with ITP had significantly higher levels of these antibodies compared to healthy dogs. This new testing method could help veterinarians better understand and manage the disease in affected dogs.
People also search for: dog bleeding disorder treatment · canine immune thrombocytopenia symptoms · dog platelet antibody test
Abstract
AbstractBackgroundCanine immune thrombocytopenia (ITP) ranges from a mild to severe bleeding disorder, and platelet counts do not reliably predict clinical disease course. The detection of platelet autoantibodies may further define the disease phenotype, but variability in assay configurations and a lack of well‐characterized controls limit the diagnostic utility of anti‐platelet antibody assays.ObjectivesWe aimed to develop control reagents to facilitate the characterization of canine platelet surface‐associated immunoglobulin (PSAIg) in flow cytometric assays.MethodsSilica microspheres were coated with canine IgG and IgM to assess the reactivity of goat and rabbit origin anti‐canine immunoglobulin reagents. They were also used as positive controls in the PSAIg assay. Preliminary assay evaluation and determination of sample stability used PRP isolated from seven healthy dogs and 26 dogs newly diagnosed with thrombocytopenia.ResultsBlood sample stability was established for up to a 48‐hour storage time. The conjugated positive control microspheres demonstrated stable fluorescent labeling over a 2‐year observation period. Rabbit and goat origin anti‐dog IgM fluorescent antibody labels reacted nonspecifically with canine IgG. Rabbit origin anti‐dog IgG antibody demonstrated greater class specificity for canine IgG than a goat origin antibody. Thrombocytopenic dogs had a broad range of membrane‐bound immunoglobulin. Median PSAIgG for dogs with primary or secondary ITP (18.4%, 34.1%, respectively) were significantly higher than controls (3.8%,P < .05).ConclusionsThe described assay reagents and procedures provide positive controls and allow consistent thresholding to define a positive test result, suitable for any flow cytometer. A rabbit anti‐dog IgG fluorescent label demonstrated specificity for canine IgG and was useful for the detection of PSAIgG in thrombocytopenic dogs.
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Search related cases →Original publication on Crossref: https://doi.org/10.1111/vcp.13093