Peer-reviewed veterinary case report
Quantitative analysis of CD3ε in a cloned canine lymphoma cell line by selected reaction monitoring assay
- Journal:
- Bioscience, Biotechnology, and Biochemistry
- Year:
- 2014
- Authors:
- Kamiie, Junichi et al.
- Affiliation:
- Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Sagamihara, Japan · Japan
- Species:
- dog
Abstract
AbstractWe established a mass spectrometry-based quantitative method of assaying CD3ε, a component of the T-cell receptor complex. It revealed a CD3ε level of 1 mol per cell in a newly derived canine T-cell lymphoma cell line. Our results suggest that this method has sufficient sensitivity to quantify CD3ε levels in canine lymphoma cells reliably.
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Search related cases →Original publication: https://doi.org/10.1080/09168451.2014.878216