Peer-reviewed veterinary case report
Quantitative real-time PCR for detection of the neurotoxin gene of Clostridium botulinum type B in equine and bovine samples.
- Journal:
- Veterinary journal (London, England : 1997)
- Year:
- 2012
- Authors:
- Johnson, Amy L et al.
- Affiliation:
- Department of Clinical Studies · United States
- Species:
- horse
Abstract
Clostridium botulinum type B is estimated to cause more than 85% of cases of equine botulism in the United States, as well as many outbreaks in cattle. In this study, a quantitative real-time polymerase chain reaction for detection of the neurotoxin gene of C. botulinum type B was compared to the mouse bioassay using 45 positive and 43 negative samples of equine, bovine or associated environmental origin. The sensitivity of the qPCR assay was 96%, whereas the sensitivity of the mouse bioassay was 84%. The specificity of the qPCR assay was 95% and the specificity of the mouse bioassay was 100%.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22537645/