Peer-reviewed veterinary case report
Rapid test for feline herpesvirus-1 infection in cats using
By Shao, Peipei et al.·Published in Veterinary research communications·2026·Guangdong Ocean University, China·View original on PubMed →
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Original publication title: Rapid and sensitive detection of feline herpesvirus-1 using fluorescent microspheres as labels for immunochromatographic test strips.
- Species:
- cat
Plain-English summary
A young cat with a runny nose and sneezing was tested for feline herpesvirus-1, a common virus that causes upper respiratory infections in cats. Researchers developed a quick test that can detect this virus in just 10 minutes using special fluorescent particles. They found that this new test matched the results of a more traditional lab test 99% of the time, making it a reliable option for diagnosing this infection. This rapid testing method could help veterinarians quickly identify and treat affected cats.
People also search for: cat sneezing treatment · feline herpesvirus symptoms · how to test for cat respiratory infection
Abstract
Feline herpesvirus-1 is a local contact-infectious pathogen that causes acute upper respiratory tract infections in cats. This virus exclusively affects felines, with a clinical incidence rate of nearly 100%, particularly in young cats. To address this health concern, this study aimed to develop a rapid fluorescence microsphere immunochromatography assay (FM-ICA) for the direct detection of Feline herpesvirus-1 (FHV-1) antigen. This method utilizes a fusion protein and fluorescent nanoparticle-labelled monoclonal antibody to detect FHV-1 within 10 min, achieving a detection limit of 2.5 × 10TCID/mL. Critically, the assay exhibited excellent specificity with cross-reactivity against canine distemper virus, canine parvovirus, canine adenovirus, canine coronavirus, feline plague virus, feline calicivirus, or feline infectious peritonitis virus. The field and clinical applicability of the method was evaluated using 100 clinical samples, including 30 faecal samples and 70 nasopharyngeal secretion samples from cats. The coincidence rate between the FM-ICA test results and the polymerase chain reaction (PCR) test results of the clinical samples was 99%.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/41779066/