Peer-reviewed veterinary case report
Rapid enzyme-linked immunosorbent assay for detecting antibodies to canine parvovirus
- Journal:
- American Journal of Veterinary Research
- Year:
- 1985
- Authors:
- Fiscus, Susan A. et al.
- Affiliation:
- From Syngene Products and Research, Fort Collins, CO 80522 (Fiscus, Mildbrand, Teramoto, Winston), and Ohio State University, Department of Veterinary Preventative Medicine, Columbus, OH 43210 (Gordon). · United States
- Species:
- dog
Abstract
SUMMARY A rapid screening assay for determining antibodies to canine parvovirus in dog serum using monoclonal antibodies and enzyme-linked immunosorbent assay (elisa) technology was developed. The elisa could be read visually, and the results correlated well with serum neutralization (sn) and hemagglutination inhibition (hi) titers. Sera with sn ≤ 1:4 or hi ≤ 1:10 had an 87.9% correlation with elisa and sera with sn ≥ 1:64 or hi ≥ 1:80 had a 94.4% correlation. The assay took only 10 to 15 minutes to perform and did not require specialized equipment. The elisa should be useful in monitoring dogs for the presence of maternal antibodies against parvovirus and for determining seroconversion after vaccination.
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Search related cases →Original publication: https://doi.org/10.2460/ajvr.1985.46.04.859