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Peer-reviewed veterinary case report

Real-time PCR detection of Brucella spp. DNA in lesions and viscera of bovine carcasses.

Journal:
Journal of microbiological methods
Year:
2014
Authors:
Sola, Marília Cristina et al.
Affiliation:
Centro de Pesquisa em Alimentos · Brazil

Abstract

This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/24978591/