Retrospective analysis of antimicrobial resistance ofspp. isolated from livestock and its environment in Thailand.

Abstract

INTRODUCTION: A retrospective study of non-typhoidalisolation from poultry and pig farms in Nakhon Pathom and Suphan Buri provinces was conducted from 2008 to 2015. The aim of study was to examine the prevalence of antimicrobial resistance and class I integrons related to gene resistance ofin livestock and its environment. METHODS: A total of 636isolates was collected from livestock and environmental samples. The isolates included 1.42%Typhimurium, 4.40%, and 1.26%; however, neithernorwere found. Allisolates was tested for antimicrobial susceptibility and minimum inhibitory concentrations (CLSI Vet03-S2 2014, NCCLS standard). RESULTS: The top three drug resistances were to cephalexin, gentamicin, and amoxicillin.Typhimurium showed resistance rates of 100%, 100%, and 22.22% to these antibiotics, respectively;showed resistance rates of 100%, 100%, and 90.91%; andrevealed resistance at the rates of 50%, 50%, 12.50%, respectively. The conserved segment integrase 1 and gene cassette were found by polymerase chain reaction (PCR) in all serotypes. The resistance gene ofb,I1,(6')-la,(6')-lb,,A,,A1, A10 and A12 were not detected fromTyphimurium and fewer resistance genes were detected when compared to the other two subtypes. DISCUSSION: These findings could be used to set up the prevention and control strategies for addressing future antimicrobial resistance of, which remains a major food safety concern.