Peer-reviewed veterinary case report
Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination.
- Journal:
- Journal of clinical microbiology
- Year:
- 2016
- Authors:
- Hwang, Ji-Hyeon et al.
- Affiliation:
- Foot and Mouth Disease Division · South Korea
Abstract
During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26560537/