Peer-reviewed veterinary case report
Saliva test to find oral melanoma and cancer in dogs
By Ploypetch, Sekkarin et al.·Published in Journal of veterinary internal medicine·2024·Department of Clinical Sciences and Public Health·View original on PubMed →
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Original publication title: Salivary metabolomic identification of biomarker candidates for oral melanoma and oral squamous cell carcinoma in dogs.
- Species:
- dog
Plain-English summary
A group of dogs with oral tumors, including oral melanoma and oral squamous cell carcinoma, were studied to find specific markers in their saliva that could help differentiate these cancers from benign tumors. Researchers found distinct differences in the levels of certain substances in the saliva of dogs with cancer compared to those with benign tumors and healthy dogs. Notably, they identified potential biomarkers like decreased levels of seryl-arginine and sarcosine in dogs with oral squamous cell carcinoma. These findings suggest that saliva tests could be a useful, non-invasive way to help vets diagnose oral cancers in dogs.
People also search for: dog oral melanoma symptoms · dog oral squamous cell carcinoma treatment · dog saliva cancer biomarkers
Abstract
BACKGROUND: Oral melanoma (OM) and oral squamous cell carcinoma (OSCC) are frequently diagnosed in dogs, presenting a challenge in distinguishing them from benign oral tumors (BN). Salivary metabolomic biomarkers offer a practical solution because of saliva's direct contact with tumors and the noninvasive nature of collection. OBJECTIVE: Assess the diversity and abundance of the salivary metabolome in dogs with BN, OM, and OSCC using amine/phenol submetabolome analysis and high-performance chemical isotope labeling liquid chromatography-mass spectrometry (CIL LC-MS). ANIMALS: Study included 11 BN, 24 OM, 10 OSCC, and 20 healthy control dogs. METHODS: Case-control cross-sectional study was conducted to assess salivary submetabolic profiles in dogs with BN, OM, and OSCC and healthy dogs. Samples were labeled withC-dansyl chloride and analyzed using CIL LC-MS targeted to amine- and phenol-containing metabolites for amine/phenol submetabolome analysis. RESULTS: Distinct clusters and significant differences in metabolite concentrations were observed among the oral cancer, BN, and control groups. A total of 154 and 66 metabolites showed significantly altered concentrations, particularly in OM and OSCC, respectively, when compared with BN (Padj < .05). Potential metabolic biomarkers were identified for each cancer, including decreased concentrations of seryl-arginine and sarcosine in OSCC. Moreover, high-confidence putative metabolites were identified, including an increase in tryptophyl-threonine and a decrease in 1,2-dihydroxynapthalene-6-sulfonic acid and hydroxyprolyl-hydroxyproline for OM. CONCLUSIONS AND CLINICAL IMPORTANCE: We identified high coverage of the amine/phenol submetabolome, including seryl-arginine, and sarcosine, in OSCC. Our findings emphasize the potential of these biomarkers for distinguishing between oral OSCC and BN in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38703129/