SDS-EDTA-treated chromatography paper strips for sampling and transportation of monkeypox virus, varicella zoster virus and herpes simplex 1 and 2 viruses.

Abstract

Mpox is a viral zoonotic disease characterized by flu-like symptoms followed by rash, which can be difficult to distinguish from other rash-presenting infections. Laboratory diagnosis is essential for confirming monkeypox virus (MPXV) infection; However, challenges in specimen transport and storage in endemic regions, especially in rural areas with limited infrastructure, hinder timely diagnosis. This study evaluates the use of SDS-EDTA-treated chromatography paper strips for testing different viral dilutions of MPXV, varicella-zoster virus (VZV), herpes simplex viruses 1 (HSV-1) and 2 (HSV-2) under different storage durations and temperatures. The results showed that all viral dilutions could be reliably detected even after 120 days at 37 °C. Notably, MPXV concentrations as low as 2 copies/µL were detected, highlighting the clinical relevance of these strips. This method could enhance mpox testing in resource-limited areas by overcoming logistical barriers, offering a cost-effective and reliable alternative for specimen collection, transport and storage in regions affected by mpox outbreaks.