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Peer-reviewed veterinary case report

Rapid simple tests to detect parvovirus in dogs

By Marulappa, Shashidhara Y. & Kapil, Sanjay·Published in Clinical and Vaccine Immunology·2009·Oklahoma Animal Disease Diagnostic Laboratory (OADDL), Center for Veterinary Health Sciences, Stillwater, Oklahoma 74078·View original on Crossref

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Original publication title: Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Canine Parvovirus-Specific Antibodies

Species:
dog

Plain-English summary

A group of 8-week-old puppies was tested for canine parvovirus (CPV), a serious virus that causes severe intestinal illness. Researchers developed two quick tests that can detect the virus in feces and measure antibodies in blood, providing results in just one minute. These tests are easy to use and can help identify CPV in puppies at risk, making it easier for veterinarians to diagnose and treat this potentially deadly infection. Early detection can lead to better outcomes for affected puppies.

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Abstract

ABSTRACT Canine parvovirus (CPV) is the number one viral cause of enteritis, morbidity, and mortality in 8-week-old young puppies. We have developed twin assays (slide agglutination test [SAT] for CPV antigen and slide inhibition test [SIT] for CPV antibody) that are sensitive, specific, cost-effective, generic for all genotypes of CPV, and provide instant results for CPV antigen detection in feces and antibody quantification in serum. We found these assays to be useful for routine applications in kennels with large numbers of puppies at risk. The results of these assays are available in 1 min and do not require any special instrumentation. SAT-SIT technology will find applications in rapid screening of samples for other hemagglutinating emerging viruses of animals and humans (influenza virus and severe acute respiratory syndrome coronavirus).

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Original publication on Crossref: https://doi.org/10.1128/cvi.00304-08