Single nucleotide polymorphism profiles of canine T-cell and null-cell lymphomas.

Abstract

BACKGROUND: The histopathological classification of T-cell lymphoma (TCL) in humans has distinctive mutational genotyping that suggests different lymphomagenesis. A similar concept is assumed to be observed in dogs with different TCL phenotypes. OBJECTIVE: This study aimed to identify the previously reported single-nucleotide polymorphisms (SNPs) in both human beings and dogs in canine TCLs and null-cell lymphomas (NCLs) and to design compatible oligonucleotides from each variant based on the multiplex polymerase chain reaction. METHODS: Genomic DNA was extracted from 68 tumor specimens (62 TCLs and 6 NCLs) and 5 buffy coat samples from dogs with TCL. Four TCL subtypes and NCL were analyzed in 44 SNPs from 21 genes using the MassARRAY. RESULTS: The greatest incidences of SNPs observed in all TCL subtypes and NCL warec.1259A > C,c.1275A > G,c.2040 + 200C > G, andc.1024C > T, respectively. Some SNP locations were statistically significant associated with NCL, includingp.S75F ( = 0.0003),p.I149N ( = 0.030),p.F37LX ( = 0.012), andp.R583* ( = 0.012). CONCLUSION: Each TCL histological subtype and NCL are likely to contain distinctive mutational genetic profiles, which might play a role in lymphoma gene-risk factors and might be useful for selecting therapeutic target drugs for each canine patient.